Epigenetics CRISPRi screen in mouse neuronal progenitor cells (NPCs). Epigenetics CRISPRi screen in mouse neuronal progenitor cells (NPCs)

This dataset examined the epigenetic gene effect on mouse NPCs with Upf2 gene knockout (KO). The sgRNA sequencing results form the epigenetics CRISPRi screen sample are reported. Overall design: CRISPR sgRNA sequencing: dCas9-Krab-expressing NPCs were harvested from the Upf2(f/f) mice, and transduced with a lentiviral CRISPRi library targeting mouse epigenetics genes (Total 3630 sgRNAs). The integrated sgRNA were amplified from each sample using DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’- CCTAGGAACAGCGGTTTAAAAAAGC-3’ for high-throughput sequencing (SE75, NextSeq550).