Tet-inducible Keratinocytes as A Novel Tool for Assessment of Anti-TNFα Activities

Detail method for construction of a Tet-inducible keratinocyte cell model for assessing TNF-α activities is shown in Methods file.  Supporting data demonstrating inducible TNF-α in the membrane fraction and secreted into the medium is shown in Figure 1.  Data demonstrating that Tet-inducible TNF-α could induce pro-inflammatory cytokines similar to recombinant TNF-α is shown in Figure 2.  Data demonstrating that our keratinocytes expressing Tet-inducible TNF-α cell model could be used to assess anti-TNFα activites is shown in Figure 3.