Genomic ddRAD datasets of Ethiopian Lophuromys - raw data.
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http://hdl.handle.net/11104/0317292
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The files contain pair-end reads that were submitted to ipyrad (Eaton & Overcast, 2020) for denovo assembly of ddRAD loci. The reads were demultiplexed and barcodes removed in Skewer (Jiang, Lei, Ding & Zhu, 2014) and pre-processing of read ends in PEAR (Zhang, Kobert, Flouri & Stamatakis, 2014). Every pair of reads was merged (with minimum required overlap of 10 nucleotides) and cut in two halves. If they were not successfully merged, we trimmed off the last 10 nucleotides from the reverse read. The PEAR-treatment was necessary to avoid poor quality mergers in a substantial proportion of loci. The data are used and analysed in Komarova et al., submitted to Molecular Ecology.
提供机构:
ASEP Repository
创建时间:
2022-06-24



