The effect of estrogen on microRNA expression in primary cultured rat neonatal cardiomyocytes. The effect of estrogen on microRNA expression in primary cultured rat neonatal cardiomyocytes

Dysregulation of miRs has been reported in a variety of cardiac diseases. In particular, it has reported that estrogen regulates a miRs network in female cardiac fibroblasts, thereby modulating a spectrum of genes involved in cardiac fibrosis and remodeling. However, the estrogen-responsive miRs in cardiomyocytes still remain to be elucidated. We used a microRNA microarray screening approach to address the miRs expression profiling in estrogen-treated cardiomyocytes. Overall design: Primary cultured rat neonatal cardiomyocytes were treated with 17beta-estradiol (E2) for 24h. Cells were then used for microRNA extraction and hybridization on Affymetrix GeneChip miRNA 2.0 Array.