Genetic markers employed for genotyping progeny from α-α unisexual and a-α bisexual reproduction.

1: The marker names and the locations are based on JEC21 chromosome 4 (GenBank: NC_006686.1). “Inter-” in the marker name indicates the marker is located in the intergenic region between the two genes in the marker name. Locations of the markers were estimated using the midpoints of the genes, or the intergenic regions between two genes, located on JEC21 chromosome 4.2: Markers SXI1Dα and SXI2Da target different alleles of the same locus of the two parental strains that underwent a-α bisexual reproduction.3: SXI1Dα and SXI2Da are PCR markers. For α-α unisexual production, marker SXI1Dα produces PCR products that show size dimorphism between parental strains 431α and XL280αSS due to the C-terminal truncation of the SXI1Dα gene in strain 431α [18], and thus, the marker is co-dominant. For a-α bisexual reproduction, markers SXI1Dα and SXI2Da are both dominant as they amplify PCR products from parental strains 431α and XL280a, respectively.4: Markers SXI2Da and Inter-CND06040–CND06050 were only used for genotyping progeny from a-α bisexual reproduction.5: SXI2Da is specific for the MATa allele and is not present in JEC21.Genetic markers employed for genotyping progeny from α-α unisexual and a-α bisexual reproduction.