Developing seeds were selected at 28 days after pollination (DAP) from L1 plants for RNA-seq analysis to investigate the potential downstream targets of BnaA01.AP2, with ‘K407’ as control. The develop
The Nudum (Nud) gene controls the caryopsis type of cereal crops by regulating lipid biosynthetic pathways. Based on the HvNud sequence and its homologous gene sequences in wheat, a conserved sgRNA wa
Additional file 2: Table S1. pegRNA target sites, RT templates and PBS sequences. Table S2. Summary of genotypes of individual plants induced by CMPE-ePPEplus in wheat plants in T0 generation. Table S