Rewiring of the Euscelidius variegatus Proteome in Response to Flavescence dorée: Insights from DDA/DIA Mass Spectrometry and de novo Genome Assembly

This study delivers the first comprehensive proteomic characterization of the insect vector Euscelidius variegatus following the acquisition of Flavescence dorée phytoplasma. Flavescence Dorée (FD) is a quarantine disease that leads to severe vine decline and yield reduction in vineyards across Europe. By integrating newly generated genomic resources with advanced data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry data, we systematically investigated the intricate molecular interactions underpinning the tripartite relationship among the insect host, its microbiome, and the phytoplasma pathogen.