RNA sequencing of parental lung cancer cells with USP18 treatment

RNA-seq data from Liu et al., 2025. A549 lung cancer cells stably expressing USP18 or empty vector treated with vehicle as a control or β-Lapachonefor 48 hours were harvested to extract total RNA for sequencing. RNA sequencing libraries were prepared using the TruSeq Stranded mRNA ligation protocol and sequenced on an Illumina NextSeq 2000 platform. Gene expression profiling from the RNA-seq data revealed differentially expressed genes and pathways following β-lapachone treatment in parental lung cancer cells, with or without gain of USP18 expression.