GTF ChIP-Seq of WT (KOS) and ICP4 null (n12) HSV-1 Productive Infection in MRC5 cells

MRC5 cells were infected with WT (KOS) or ICP4-null HSV-1 (n12) at an MOI of 10 PFU/cell for 2.5 hours and ChIP-Seq for ICP4, Pol II, TBP, Sp1, Med1, and Med23 was performed. Illumina libraries were constructed for the IP and input material. Illumina sequencing was carried out at Tufts University Core Facility using HiSeq 2500 to generate 50 bp single-end reads.