Rescue of ptpn11a mutant phenotype by injection of ptpn11a and ptpn11b.

Clutches of embryos from ptpn11a+/-ptpn11b-/- or ptpn11a+/-ptpn11b+/+ zebrafish were split and injected with ptpn11a mRNA or ptpn11b mRNA. The embryos were scored based on their morphology at 5 dpf. Phenotype represents the number of embryos with mutant morphology/total number of embryos. Subsequently, the genotype was determined by sequencing and is depicted as number of ptpn11a-/- embryos/total number of embryos analyzed. Ptpn11b was homozygous (ptpn11b-/- or ptpn11b+/+) and was not determined in these embryos.