Demultiplexed 16S rRNA Nanopore FASTQ Sequences of Black Soldier Fly Gut Microbiota Using 27F/1492R Primers

This dataset contains demultiplexed 16S rRNA gene sequences generated using Oxford Nanopore sequencing from gut microbiota of Hermetia illucens (black soldier fly) larvae. The V1–V9 region of the 16S rRNA gene was amplified using universal bacterial primers 27F and 1492R prior to sequencing. Samples were collected from multiple generations and sub-lines of black soldier fly larvae reared on a novel substrate as part of a study investigating transgenerational microbial dynamics. All samples were processed using a standard barcoded library preparation protocol, and basecalled reads were demultiplexed using Guppy. The uploaded FASTQ files represent raw, demultiplexed reads for each sample and are suitable for downstream microbial community analysis such as taxonomic classification and diversity profiling.