Biomarkers for Down Syndrome screening in murine fetal liver and placenta

The Ts1Cje mouse strain (Sago, 1998) contains a segmental trisomy of mouse chromosome 16 orthologous to the region of human chromosome 21 commonly associated with Down Syndrome. In this study, fetuses were obtained from wildtype mothers bred with either wildtype or Ts1Cje males. Gene expression profiles in fetal liver and placenta of wildtype and Ts1Cje fetuses were compared, to identify potential markers for application in human prenatal DS screening. A 48 array study with 24 arrays for placenta and 24 for fetal liver. For each tissue we used RNA from 24 individual embryos, i.e. six male and six female embryos from both genotypes.Ts1Cje小鼠品系（Sago等，1998）携带小鼠16号染色体的片段三体性变异，该染色体区段与人类21号染色体中通常与唐氏综合征（Down Syndrome）相关的区域同源。本研究的胎鼠样本来自野生型母鼠与野生型或Ts1Cje公鼠的交配子代。研究比较了野生型与Ts1Cje胎鼠的胎肝和胎盘组织的基因表达谱，旨在筛选可应用于人类产前唐氏综合征筛查的潜在标志物。本研究共使用48张基因芯片（array），其中24张用于胎盘样本，24张用于胎肝样本。对于每种组织，我们均使用24个独立胚胎的RNA，即两种基因型（野生型、Ts1Cje）各包含6只雄性和6只雌性胚胎。