soil metagenome Genome sequencing

Microbial ecological mechanisms on carbon and nitrogen interaction in a black soilThe intensive use of nitrogen (N) fertilizer has induced serious environmental pollution in China. In order to decrease the usage of N, some substitutes should be promoted. Crop residue contains plenty of N and was usually removed after harvesting. It was widely accepted that the application of crop residue would ameliorate soil fertility. However, the interaction of residue carbon (C) and mineral N was poorly understood. In the present study, a black soil in northeast China was selected. Maize stalk was added with or without N fertilization, then different soil N pools and soil microbial community structure were invested during the growth season. Soil microbial community structure would be analyzed by Illumina HiSeq 2500 sequencing platform, and their linkages with soil N pools would be evaluated. The in-depth understanding of the microbial ecological mechanisms on carbon and nitrogen interaction in a black soil, will provide the sound scientific support for reducing the usage of N fertilizer.