Cloning of an albino mutant of Arabidopsis thaliana using mapping by sequencing

We used mapping-by-sequencing to identify a point mutation that damages a conserved residue at the acceptor site of an intron of the At2g04030 gene of Arabidopsis thaliana, which encodes the chloroplast-localized AtHsp90.5 protein, a member of the HSP90 family of heat-shock proteins.