16S sequencing of mouse gut microbial DNA after 10 days of intermittent fasting or ad libitum with and without vancomycin treatment

To compare the changes in the gut microbial composition in the cecum of mice following the 4 treatments (IFvsAL with(out) vancomycin co-treatment) Overall design: Mice (6-8 weeks) underwent following procedures: a) 10 days of ad libitum food supply b) 10 days of intermittent fasting c) 10 days of ad libitum food co-treated with vancomycin in the drinking water (50mg/kg/day; 214.27 mg/l) d) 10 days of intermittent fasting food cotreated with vancomycin in the drinking water. The cecum content was removed and microbial genomic DNA extracted.