Rescue of mutant phenotypes with recombineered fosmids.
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*See Figure 6 and 7 for constructs. che-1 rescue was assessed by injecting the recombineered fosmid into mutants ot94; ntIs1 (gcy5prom::gfp) that display a 100% penetrant total loss of gfp expression. Rescued animals restore gfp expression in ASER. lsy-2 rescue was assessed by injecting recombineered fosmids into ot90; ntIs1 (gcy5prom::gfp) that display 100% penetrant ectopic gfp expression in ASEL. Rescued animals show gfp expression only in ASER. For each line at least 20 L4 worms were scored.1100% of animals show rescue.2>50% of animals show rescue.3No rescue and no reporter expression.
创建时间：
2015-12-02
