Dataset of changes in viscosity of Escherichia albertii cultures during storage at room temperature

Escherichia albertii was named in 2003 as a new species in the genus Escherichia and is known as a causative agent of diarrhea. Although the biochemical characteristics of E. albertii are not specific for differentiating it from other species, it is now possible to identify the species by PCR targeting specific genes. Among about 100 strains of E. albertii at our institution, only one strain was viscous, but we examined the change in viscosity because some of the strains acquired viscosity during storage. The target strain was the strain with viscosity change, and the controls were the viscous strain, E. albertii JCM strain, and E. coli ATCC strain. Biochemical properties, drug susceptibility, and string tests were performed under various conditions. No significant differences were observed in biochemical properties and drug susceptibility tests. In the string test of the viscous strain, the viscous colonies did not show any significant change in viscosity with passaging, especially the non-viscous colonies did not show any change in viscosity. Similarly, there was no change in the viscosity of either the viscous or the non-viscous colonies by passaging in the strain with altered viscosity. Neither the length of the storage period nor the casitone concentration affected the viscosity change. The change in viscosity was related to the storage of the strain at room temperature, suggesting that the process from the clinical specimen to the storage after isolation may have affected the change in viscosity.

大肠埃希菌阿尔贝蒂（Escherichia albertii）于2003年被命名为埃希菌属中的一个新物种，并被确认为引起腹泻的致病菌。尽管该菌的生化特征并不能特异性地区分其与其他物种的差异，但目前通过针对特定基因的聚合酶链式反应（PCR）技术已能够对该物种进行鉴定。在我机构所持有的约100种E. albertii菌株中，仅有一种菌株表现出粘稠性，然而，我们仍对其粘稠性的变化进行了研究，因为部分菌株在储存过程中获得了粘稠性。目标菌株为粘稠性发生变化的菌株，对照菌株则包括粘稠菌株、E. albertii JCM菌株以及大肠杆菌ATCC菌株。在多种条件下，我们对菌株的生化特性、药物敏感性和菌落特征进行了检测。在生化特性和药物敏感性测试中，并未观察到显著差异。在对粘稠菌株进行的菌落特征测试中，粘稠性菌落随着传代并未显示出显著的粘稠度变化，尤其是非粘稠性菌落亦未观察到粘稠度的变化。同样，粘稠性和非粘稠性菌落经过传代后，其粘稠度亦未发生变化。储存时间的长短以及乳糖蛋白胨浓度的变化均未对粘稠度的改变产生影响。粘稠度的变化与菌株在室温下的储存状态有关，这表明从临床样本到分离后的储存过程可能对粘稠度的变化产生了影响。