Data from: Influence of factors of cryopreservation and hypothermic storage on survival and functional parameters of multipotent stromal cells of placental origin

Human placenta is a highly perspective source of multipotent stromal cells
(MSCs) both for the purposes of patient specific auto-banking and
allogeneic application in regenerative medicine. Implementation of new GMP
standards into clinical practice enforces the search for relevant methods
of cryopreservation and short-term hypothermic storage of placental MSCs.
In this paper we analyze the effect of different temperature regimes and
individual components of cryoprotective media on viability, metabolic and
culture properties of placental MSCs. We demonstrate (I) the possibility
of short-term hypothermic storage of these cells; (II) determine DMSO and
propanediol as the most appropriate cryoprotective agents; (III) show the
possibility of application of volume expanders (plasma substituting
solutions based on dextran or polyvinylpyrrolidone); (IV) reveal the
priority of ionic composition over the serum content in cryopreservation
media; (V) determine a cooling rate of 1°C/min down to -40°C followed by
immersion into liquid nitrogen as the optimal cryopreservation regime for
this type of cells. This study demonstrates perspectives for creation of
new defined cryopreservation methods towards GMP standards.