Temporal Transcriptomic Profiling of Lettuce in Response to Endophytic Bacterial Inoculation

This study explores the dynamic transcriptomic response of lettuce (Lactuca sativa cv. Green Oak Leaf) to inoculation with the endophytic bacterium Pseudomonas psychrotolerans strain IALR 632. RNA-seq was performed on 24 samples collected at three time points: 4, 10, and 15 days after transplanting (DAT) to capture temporal changes in gene expression associated with beneficial plant-microbe interactions. Differentially expressed genes were identified relative to untreated controls, providing insights into host regulatory mechanisms, stress responses, and potential growth-promoting effects induced by endophytic colonization.