MOESM1 of Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands

Additional file 1. a Flow cytometric analysis of M2 macrophages (CCR2-/Galectin3+) in the PI-/CD11b+ cell fraction of PBMNCs (Day 0) and E-MNCs (Day 5). CCR2-/Galectin3+ cells occupied approximately 50% of the CD11b positive cell fraction in E-MNCs. High levels of intracellular galectin 3 expression are considered essential for transcriptional activation towards M2 macrophages after M1. b Flow cytometric analysis of T helper cells (CD3+/CD4+) in the PI fraction of PBMNCs (Day 0) and E-MNCs (Day 5). CXCR4+/CXCR6− cells (Th2) were approximately 20% of the CD3- and CD4-positive T helper cell fraction of E-MNCs. Th1, CXCR3 positive cells in T helper cells; Th17, CXCR4 and CXCR6 positive cells in T helper cells. c Representative pictures of EPC-CFU (Scale bar: 100 μm) at 7 days of EPC-CFA (100×), and the right panel shows ILB4-conjugated FITC binding and AcLDL-DiI uptake of each EPC-CFU (Scale bar: 100 μm) (40×). d Percentage of endothelial stem/progenitor cell fraction (c-kit+/Sca-1+/lineage−) in PBMNCs and E-MNCs.