Transcriptomic analysis of the diethylnitrosamine (DEN)-induced HCC mRNA from Pck1 KO and WT mice

To investigate the role of hepatic Pck1 in the diethylnitrosamine (DEN)-induced HCC, a Pck1 knockout mouse in the liver was developed with Alb-Cre.HCC was induced in mice by combined treatment with DEN (75 mg/kg) and CCl4 (2 mL/ kg, twice per week for 12 weeks).At 32 weeks, the mice were sacrificed after fasting for 12 hours, and liver tissues with tumors were collected for examination. RNA was extracted from the livers of mice from all treatment groups and used for RNA seqencing. RNAseq data demonstrated that a lack of Pck1 can play an important role in the development of HCC. Use RNA-sequencing to investigate the role of Pck1 inthe diethylnitrosamine (DEN)-induced HCC on a transcriptomic level, by comparing the HCC of WT and Pck1-null mice using Illumina technology.