Parameter values estimated by the proposed Bayesian approach using the experimental SPR response data.

Three tests were carried out. In the first experiment, the interaction data between CAII and 4-CBS were studied on a SensiQ Pioneer biosensor under a partial mass-transport limited condition. The data were analyzed and the resulted kinetic parameters and active concentration. In the other two tests, two datasets were collected from the literature. Dataset #1 was from the work done by Castonguay et al.[32], in which BMP10 interacted with immobilized human EngECD-Fc in a Biacore 3000 system. Dataset #2 was from a different work done by Abdiche et al.[33], in which human CGRPα bound immobilized 4901 IgG in a Proteon XPR36 biosensor. The two datasets were then analyzed by the proposed approach to estimate the active concentration of analyte A0, rate constants ka and kd, and the dissociation constant KD. The returned parameters as well as the parameters estimated in the original reference papers were listed. The 95% Bayesian credible intervals for the estimation were also provided in the square brackets. The biosensor used to collect the experimental data was indicated in the parentheses.
Parameter values estimated by the proposed Bayesian approach using the experimental SPR response data.