Epithelial zonation along the mammalian small intestine defines five discrete metabolic domains [Mouse_scRNA-seq II]

Longitudinal zonation of epithelial cells across the mammalian small intestine defines five domains of nutrient absorption. Sorted epithelial cells and intestinal progenitor cells (intestinal stem cells and transit amplifying cells) were collected from 30 equal segments across the full length of 2 murine small intestines, barcoded using MULTIseq technology, pooled, and sequenced.哺乳动物小肠上皮细胞的纵向分带模式界定了五类营养吸收区域。研究人员从2条小鼠小肠全长等分的30个节段中，收集了分选后的上皮细胞与肠祖细胞（肠干细胞及转运扩增细胞），随后通过MULTIseq技术完成条码标记、混合后开展测序。