Predicted Fur binding sequences within the promoter regions of <i>N. gonorrhoeae</i> iron-induced genes.¹

1Iron regulation as previously determined by microarray analyses of N. gonorrhoeae grown in defined medium CDM (-Fe) or CDM with 10 µM ferric nitrate (+Fe) [50], [51]. 2Fur regulation was determined by comparing mRNA levels in N. gonorrhoeae WT, fur mutant and fur complemented strains grown under iron-replete and iron-deplete conditions at 1h after addition of iron and desferal using qRT-PCR. NR; not regulated by Fur. Activated; mRNA level of the gene was decreased in the fur mutant strain under either iron-replete or iron-deplete conditions compared to that in the wild type strain. Repressed; mRNA level of the gene was increased in the fur mutant strain under either iron-replete or iron-deplete conditions compared to that in the wild type strain. NE, not examined; transcriptional regulation was not tested in this study. 3Fur regulation of the genes as previously determined [8]. 4In vitro Fur binding to the 500 bp upstream sequence of ATG of these genes was determined using EMSA and foot printing in previous studies [8].