Global gene-expression profiles of wild type (WT; Columbia-0; Col) and hag1 in the course of de novo shoot regeneration

Global gene-expression profiles of WT and hag1 were generated by RNA seq to understand the role of HAG1 in de novo shoot regeneration. Overall design: WT and hag1 roots (R), 4 d CIM-incubated root explants (C), 4 d CIM-incubated + 2 d SIM (S)- and 2 w SIM (S2W)-incubated root explants were collected. Total RNA was isolated (OD260/280 ratio of 1.8 to 2.2) and RNA libraries were prepared by Beijing Genomics Institute (BGI) using standard Illumina protocols.