Processed Seurat RDS objects for retinal scRNA-seq (NRL knockdown; Figure 5)
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This record provides processed Seurat objects (RDS) used to reproduce the single-cell RNA-seq analyses shown in Figure 5 of:Lee, H., Jang, H., Chae, J.B. et al. In vivo efficacy of NRL knockdown with cell-penetrating siRNA in retinal degeneration. Scientific Reports (2025). https://doi.org/10.1038/s41598-025-07299-6 Raw sequencing data and processed files are available at GEO under accession GSE266377.Code to generate the Figure 5 panels is available on GitHub (https://github.com/hklee8668/NRL-knockdown.git). File descriptions and key metadata: celltype.txt — Cell type annotation table used to label Seurat clusters. final_Dimension_15_Resolution_0.6_celltype.RDS — Seurat object containing all major retinal cell types. celltype — cell type annotation Photoreceptor_subclustering_final_Dimension_10_Resolution_0.4.RDS — Seurat object after photoreceptor subclustering.



