Synergy volume of double and triple combinations of antivirals against 2009 H1N1 viruses A/California/04/09 (CA04), A/California/05/09 (CA05), and A/California/10/09 (CA10) as determined by Neutral Red assay.

Synergy volumes represent the sum of the synergy or antagonism across all concentrations of two drugs for a double combination, or all concentrations of two drugs at a fixed concentration of the third drug for the TCAD regimen. The concentrations of each drug used are provided in Table S1. Combinations with synergy volumes >100 µg/mL2% for double combinations or >100 µg/mL3% for triple combinations are considered to be strongly synergistic (using the lower 95% confidence interval). Conversely, combinations with synergy volumes <−100 µg/mL2% or µg/mL3% are considered to be strongly antagonistic. Bold numbers denote volumes that are defined as strongly synergistic or antagonistic. Synergy volumes are presented as the mean between replicates with 95% confidence intervals. Typically, between 3 and 6 experiments were run for each combination, with each experiment having 3 replicate wells for each condition.