Small RNAs during seed development in large-seeded chickpea

In this study, we sequenced small RNAs at seven successive stages of seed development and leaf tissue in a large-seeded chickpea cultivar (JGK 3) using Illumina platform. More than 500 million reads were generated in all the samples combined together with an average of 34 million reads in each sample. Data obtained in FASTQ files were pre-processed and unique reads representing small RNAs were identified at different stages of seed development. Total RNA was isolated from the seed stages and leaf tissues followed by preparation of small RNA libraries and sequencing using Illumina platform. The reads were pre-processed using modified perl scripts. After quality control, the identical reads were collapsed into a unique read and read count for each sequence was recorded. All the filtered unique reads from each sample were mapped on the chickpea genome to find their location.