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P. aeruginosa PA14 WT in LB medium vs in LB with 10 mM adenosine medium. Pseudomonas aeruginosa

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To investigate the impact of adenosine on gene expression of wild-type PA14. Overall design: To investigate the impact of adenosine on gene expression of wild-type PA14, cells were harvested after incubating for 7 h in LB medium and LB with 10 mM adenosine medium, and RNA was extracted with the RNeasy Mini Kit (Qiagen, Valencia, CA) using a bead beater (Biospec, Bartlesville, OK ) with RNAlater buffer (Applied Biosystems, Foster City, CA) to stabilize the RNA.

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2011-06-01
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