aAverage number of non-polar hydrogen atoms per amino acid residue.bTemperature at which denaturation is minimized (K).cTemperature at which growth is maximized (K).dThe lower temperature at which the
Each melting curve is fit by a nonlinear regression analysis using the Boltzmann function (GraphPad Prism) and the Tm value is determined by the inflection point of the transition curve as defined fro
