Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [NKT_after_Dex]

The goal of this study was to understand the role of thymic natural killer T cells (NKT) in thymic regeneration following dexamethasone-induced acute involution. For this purpose we treated WT mice with dexamethasone, sorted NKT from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA Seq. Overall design: Young male WT mice were treated with dexamethasone 3 days/18 hours before sacrifice, and then thymic NKT were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 4-5 replicates were used. The control was 3 days vehicle-treated age-matched WT mice.