Supplementary data files
收藏资源简介：
We measured N<sub>2</sub> fixation along the Western Antarctic Peninsula (WAP) and at Palmer Station over two austral summer months. N<sub>2</sub> fixation rates measured by <sup>15</sup>N<sub>2</sub> assays were below conservative detection limits, despite being detected when using less stringent detection thresholds, suggesting a potential for N<sub>2</sub> fixation. This was confirmed by our continuous acetylene reduction method (FARACAS). <i>nifH </i>gene amplicon sequencing revealed that Gammaproteobacteria were the dominant class of identified diazotrophs in the WAP’s coastal waters. However, Epsilonproteobacteria contributed disproportionally to <i>nifH</i> expression, particularly when putative N<sub>2</sub> fixation rates were at the highest. Combining diazotroph communities with environmental observations, we hypothesize that vertical water mixing resuspended sediments into the water column and contributed to the limited N<sub>2</sub> fixation in Antarctic waters.
