matrix of SRAP marker data

We scored seven primer combinations o SRAP-primers, namely ME1-EM1; ME1-EM3; ME3-EM1; ME3-EM3; ME3-EM4; ME1-EM5; ME3-EM5. Forward primers me1, 5'-TGAGTCCAAACCGGATA-3'; me2, 5'-TGAGTCCAAACCGGAGC-3'; me3, 5'-TGAGTCCAAACCGGAAT-3'; me4, 5'-TGAGTCCAAACCGGACC-3'; me5, 5'-TGAGTCCAAACCGGAAG-3'. The reverse primers were em1, 5′-GACTGCGTACGAATTAAT-3′; em2, 5′-GACTGCGTACGAATTTGC-3′; em3, 5′-GACTGCGTACGAATTGAC-3′; em4, 5′-GACTGCGTACGAATTTGA-3′; em5, 5′-GACTGCGTACGAATTAAC-3′. The resulting PCR products were checked on 2% agarose gel and visualized (after staining with ethidium bromide) under UV-light. To determine the length of DNA fragments a marker of known molecular weight (100 bp DNA Ladder) was run in parallel.