Genome Mining of a Prenylated and Immunosuppressive Polyketide from Pathogenic Fungi

Activation of the polycyclic polyketide prenyltransferase (pcPTase)-containing silent clusters in Aspergillus fumigatus and Neosartorya fischeri led to isolation of a new metabolite neosartoricin (3). The structure of 3 was solved by X-ray crystallography and NMR to be a prenylated anthracenone. 3 exhibits T-cell antiproliferative activity with an IC50 of 3 μM, suggestive of a physiological role as an immunosuppressive agent.

在烟曲霉（Aspergillus fumigatus）和鱼藤镰刀菌（Neosartorya fischeri）中，多环聚酮烯基转移酶（polycyclic polyketide prenyltransferase，简称pcPTase）所含沉默簇的激活导致了新型代谢物鱼藤霉素（neosartoricin，3）的分离。通过X射线晶体学和核磁共振（NMR）解析，3的结构被确认为一个烯基化的蒽醌。3展现出对T细胞的抗增殖活性，其IC50值为3微摩尔，暗示其在免疫抑制剂中的生理作用。