FRT thyroid cells: 2D monolayers vs. 3D follicles

Transcriptional profiling of the Fischer Rat Thyroid (FRT) cells comparing polarizing cells grown as a confluent two-dimensional monolayer (2D culture system) with cells grown in matrigel where they acquire a three-dimensional follicular structure (3D culture system).The goal was to identify regulators of 3D epithelial thyroid polarization and follicle formation. Two condition experiment, 3D vs. 2D FRT cell cultures. Biological replicates: 4 for both conditions. A pool of the 2D replicates was compared with each 3D replicate. One dyeswap replicate comparing 2D pool with 3D pool.