CIL:36893, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library
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资源简介：
This is IMAGE #11 (second time-lapse set, 50 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The images were acquired with ~10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions.
These cells were transfected ~24 hours earlier with in vivo electroporation of plasmid DNA encoding eYFP. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.
Images were acquired a 0, 10, 20, 30, and 50 minutes. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~4 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-16
