Metatranscriptomic changes in propionate pathway and fiber degrading enzymes revealed the rebalancing mechanism of rumen microbiota after CH4 inhibition by 3-NOP in vitro

A more comprehensive understanding is needed on the rebalancing process of rumen microbiota after the addition of 3-NOP. We chose a fully automated fermentation system in vitro to observe the process of 3-NOP inhibiting methane, and extracted the fermentation fluid (n=12) at the beginning of net hydrogen consumption(24 h) for metatranscriptomic sequencing, as well as quantitative analysis of key microorganisms. Pure bacterial inhibition experiments were also conducted on four different nutritional types of methane bacteria.