Silencing of the stress granule protein TIA1 increases p-syn.

Shown are results wherein SMG1 and TIA1 were knocked down either individually or in combination to assess effects on α-synuclein. Expression levels of p-syn and t-syn for each siRNA condition normalized to the non-silencing (NS) siRNA control are shown beneath each lane on the westerns. Results are representative of at least three independent replicates. Antibodies used were as in Figure 3, with anti-TIA1 antibody (α-TIA1) included. siRNAs used are indicated across the top of the westerns.