Genome sequencing/assembly and genetic diversity of Heterocephalus glaber

Naked mole-rats (Heterocephalus glaber) are a species of rodent endemic to the horn of Africa, notable among mammals for their long lifespans, resistance to a variety of stresses, and eusocial mating behavior.  Though their natural species range extends across large portions of Kenya, Ethiopia, Somalia, and Djibouti, the vast majority of genetic and genomic analyses focus on Kenyan specimens.  Here, we constructed a chromosome-scale reference genome assembly for H.glaber, then leveraged it, along with modern whole-genome sequencing, to characterize the genetic diversity of specimens deriving from Kenya, southern Ethiopia, and eastern Ethiopia.  We found the Kenyan and southern Ethiopian specimens to be closely related to each other and highly diverged from eastern Ethiopian specimens.  We also found specimens collected from nearby locations in southern Ethiopia to be more closely related to Kenyan specimens than to each other, emphasizing the importance of local migr..., , # Genome sequencing/assembly and genetic diversity of Heterocephalus glaber

Dataset DOI: [10.5061/dryad.m37pvmdf4](10.5061/dryad.m37pvmdf4)

## Description of the data and file structure

This study presents an assembly of the genome for *H.glaber*, along with gene annotations and alignment to other mammalian genomes. It also provides polymorphism data from animals collected from multiple geographic regions.

### Files and variables

#### File: SuppTables1-8.xlsx

**Description:** An Excel file with all Supplemental Tables:

Supplemental Table 1: Genome assembly statistics across assembly steps. This table provides a detailed breakdown of assembly metrics at each stage of the genome assembly pipeline.

Supplemental Table 2: Groups of super-scaffolds, compiled using chromosome-sorted sequencing (as depicted in Figure 1A). This table lists the organization of scaffolds into chromosome-scale groupings, based on chromosome sorting and sequencing.

Supplemental Table 3: Statistics for the...,