Infection and feeding source determination.

We used two methods to determine Trypanosoma cruzi infection in bugs: direct examination (Dir-Inf) and PCR. Ni, is the number of positive individuals; Nf, the total number of examined individuals. Parasite characterization (TCII/TCI) was determined by PCR (see Materials and Methods). The number of feeding sources detected is indicated and their putative affiliation (putative feeding source) using BLAST searches. The Id (%) represents the identity of the sequences obtained from vector guts with those in GenBank (GB), with the GB code in the last row. Feeding sources detected in vectors infected by T. cruzi via PCR are in bold.