Transcriptomic RNA sequencing of Euglena gracilis with rRNA depleted

Total RNA for RNA-seq was extracted and ribosomal RNA was removed using specific probes. A strand-specific library was constructed by synthesizing first strand cDNA with random hexamer primer and M-MuLV transcripase (RNase H-) and second strand cDNA with DNA polymerase I and RNase H. The qualified library was sequenced on a Illumia Nova 6000 platform by Novogene (Beijing, China). This transcriptomic RNA sequencing is used for expression analysis of protein-coding genes in nuclear and organellar genomes.