Data and code from: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

The mechanisms underlying the generation of the immense diversity of neuronal cell types remain a fundamental question of developmental biology. In the spinal cord, different “cardinal classes” of neurons that share a common molecular identity are produced from spatially segregated progenitor domains. Within many such classes, a stereotyped sequence of divergent neuronal types of related function is generated over time, raising the question of the molecular mechanisms that control this process. Here, we show that the successive expression of mouse transcription factors Onecut2, Pou2f2, and Pou3f1 within the cardinal classes giving rise to motor and sensory circuits, correlates with the emergence of sequentially generated subpopulations of neurons within those domains. We demonstrate that the genetic loss of Pou2f2 results in impaired development of two early-born motor neuron columns and re-specification of anterolateral system projection neurons as a later-born subset. Similarly, we sh..., , , # Data and code from: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

Dataset DOI: [10.5061/dryad.j3tx95xtz](https://doi.org/10.5061/dryad.j3tx95xtz)

## Description of the data and file structure

This repository contains all data and the R code to reproduce the plots in Figures 1-7 and S1-S9 + S15 from Caudet Segarra et al. 2025.

### Files and variables

#### Data files hosted by Dryad

* Input_data_Caudet_Segarra_et_al.zip

#### Software files hosted by [Zenodo](https://zenodo.org)

* Supplemental_Experimental_Procedures_-_Imaging_Revision.Rmd
* Supplemental_Experimental_Procedures___Revision_scRNAseq.Rmd

### Supplemental files hosted by [Zenodo](https://zenodo.org)

* Output_Caudet_Segarra_et_al.zip

**Description:** Please unpack Input_data_Caudet_Segarra_et_al.zip file and download the R scripts Supplemental_Experimental_Procedures_-_Imaging_Revision.Rmd and Supplemental_Experimental_Procedures___Revision_scRNAs...,