High abundance of lactobacilli in the gut microbiome of honey bees during winter

Honey bee gut microbiota play specific roles in promoting host growth and physiology by regulating the immune system, behavior, metabolism, and neurological processes. While the gut microbiota of honey bee queens, workers, and larvae has been extensively studied, less is known about the composition of gut microbiota in the winter worker bees. This study investigates the dynamics of the gut microbiota in overwintering adult worker bees, focusing on two commercial bee strains: Bolton™ bees and Mann Lake™ bees. These Apis mellifera strains were investigated under different storage conditions (indoor storage at 6°C and outdoor storage in natural conditions) during the winter months (October, November, and December). Utilizing 16S rRNA gene amplicon sequencing, we characterized the microbial composition of the whole gut. We observed the Lactobacilli dominated in all the overwintering honey bee guts with a significantly higher abundance of unclassified Lactobacillus species in November, while..., , , # Data from: High abundance of lactobacilli in the gut microbiome of honey bees during winter

[https://doi.org/10.5061/dryad.rxwdbrvjf](https://doi.org/10.5061/dryad.rxwdbrvjf)

All .qzv file must be downloaded and uploaded to [https://view.qiime2.org/ (opens in new window)](https://view.qiime2.org/) 

**alpha_rarefaction(2).qzv:** represents alpha rarefaction- sequences

**16Staxabarplots_clean2.qzv:** Relative abundances of the bacteria presented in Honey bee adults

**16s_MAPs_GB_H (1).txt:** represents metabarcode sequences used for sequencing

**stats-dada2.qzv:** file represents filtered, denoised, merged and non chimeric reads

**unweighted_unifrac_emperor.qzv:** A QIIME 2 visualization file displaying beta-diversity analysis using the unweighted UniFrac metric, which measures phylogenetic distances between microbial communities based on the presence or absence of taxa, visualized as an Emperor plot for sample clustering.

**jaccard_emperor.qzv:** A QIIME 2 visualization file co...,