Effects of anti-PD-L1 on the testis transcriptomes of C57BL/6 mice

To explore the mechanisms of the decreased testosterone after anti-PD-L1 treatment in mice, we performed RNAseq transcriptome analysis of the testes of male mice 7 days later post-treatment with anti-PD-L1 or control IgG. After quantified by TBS380, paired-end RNA-seq sequencing library was sequenced with the Illumina HiSeq xten. Our results show that pathways involved in both lipid transportation and inflammation were obviously affected by anti-PD-L1 treatment. Overall design: Testis mRNA profiles of C57BL/6 male mice after twice adminstarion of anti-PD-L1 or IgG isotype control.