Evaluation of the Performance of Lipidyzer Platform and Its Application in the Lipidomics Analysis in Mouse Heart and Liver

Lipids
play important roles in cell signaling, energy storage,
and as major structural components of cell membranes. To date, little
work has been conducted to show the extent of tissue specificity of
lipid compositions. Here, the recently acquired Lipidyzer platform
was employed in this pilot study: (i) to assess the performance of
the Lipidyzer platform, (ii) to explore lipid profiles in liver and
cardiac tissue in mice, (iii) to examine sex-specific differences
in lipids in the liver tissue, and (iv) to evaluate biological variances
in lipidomes present in animals. In total, 787 lipid species from
13 lipid classes were measured in the liver and heart. Lipidomics
data from the Lipidyzer platform were very reproducible with the coefficient
of variations of the quality control (QC) samples, ∼10%. The
total concentration of the cholesterol esters (CE) lipid class, and
specifically CE(16:1) and CE(18:1) species, showed sex differences
in the liver. Cardiac tissue had higher levels of phospholipids containing
docosahexaenoic acid, which could be related to heart health status
and function. Our results demonstrate the usefulness of the Lipidyzer
platform in identifying differences in lipid profile at the tissue
level and between male and female mice in specific tissues.