Elucidation of the molecular mechanisms in the early response to purine starvation.
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L. donovani cell lines were generated as described in the Materials and Methods and Table 2. Changes in Fluc activity and mRNA abundance (qRT-PCR Fluc), and mRNA abundance of the corresponding endogenous allele (qRT-PCR Gene) following 6 h purine starvation were determined in parallel from aliquots of the same culture. All qRT-PCR data were normalized to UMPS. The mean and standard deviation determined from two independent biological replicates is shown for each analysis.
创建时间：
2015-12-02
