RNAseq study of organs of Ixodes ricinus ticks harbouring or not the endosymbiont Midichloria mitochondrii

Compared transcriptomes of pools of organs (ovary, salivary glands or Malpighian tubules), from two strains of Ixodes ricinus, respectively with and without the symbiont Midichloria mitochondrii. Samples were obtained by pooling organs from n=5 adult female ticks. Two different engorgement time points (D0 i.e. unfed; D4 i.e. partially fed) were compared. RNA was extracted and the transcriptome was sequenced for each library, in order to identify differentially expressed genes between the different conditions.