遇见数据集

Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells

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NIAID Data Ecosystem2026-03-07 收录
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genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed and processed for ChIP once arrested as large-budded cells. Immunoprecipitated DNA and total genomic DNA not subjected to immunoprecipitation were competitively hybridized to Nimblegen whole genome arrays.

创建时间:
2012-03-21
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