The complete chloroplast genome assembly of <i>Commelina caroliniana</i> Walter (Commelinaceae)

We sequenced and published the chloroplast genome of <i>Commelina caroliniana</i> Walter, which was previously misidentified as <i>C. diffusa</i> owing to their morphological similarities until 1989. The genome of <i>C. caroliniana</i> is 160,857 bp long [large single copy region: 88,064 bp; a small single copy region: 18,549 bp; two inverted repeat regions: 27,122 bp] and has a GC content of 35.7%. The genome comprises 133 genes, including 87 coding sequences (CDSs), 38 tRNAs, and eight rRNAs. The phylogenetic relationship between <i>C. caroliniana</i> and related species was analyzed using the maximum likelihood method based on the 79 CDSs of the chloroplast genome. Phylogenetic analysis revealed that <i>C. caroliniana</i> is closely related to <i>C. communis</i>. Our findings will contribute to studies on species identification and phylogenetic and evolutionary research. These results enhance our understanding of the <i>Commelina</i> genus.