RNA sequencing for RWPE1 cells, OLFM4-Active RWPE1 cells and OLFM4-KO RWPE1 cells

OLFM4 gene expressing in RWPE1 stem/progenitor-like cells. To study OLFM4 gene functions and molecular mechanisms, we generated OLFM4-avtive and OLFM4-knock out-GFP reporter RWPE1 cells with CRISP-Cas 9 system. The cell transcriptome was studied with RNA sequencing. Overall design: Comparision of gene expressing signature between OLFM4-knock out RWPE1 and OLFM4-wild RWPE1 cells; OLFM4-Active RWPE1 cells and OLFM4-wild RWPE1 cells.